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Old 12-02-2016, 06:42 AM   #1
dlv04c
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Location: Tallahassee, FL

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Default Bioanalyzer RNA Nano with total RNA - High MW peak above 28S?

I'm getting a significant high MW peak on a nano RNA chip with total RNA. Any thoughts on what this might be caused by? It is present in all the total RNA samples I loaded on this chip.


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Old 12-06-2016, 11:39 PM   #2
Amar
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Possibly genomic DNA. Have you digested your DNA and if so with what?

Some good info here:
http://www2.jabsom.hawaii.edu/genomi...rpretation.pdf
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Old 12-07-2016, 10:40 AM   #3
dlv04c
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Default it was not denatured

Turns out the RNA was not denatured prior to loading on the chip.
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