I am running my transcription factor chip seq samples in Bioanalyzer HS and getting weird inverted peaks(pic attached)in input samples!! has anybody come across such 'peaks' before?
These are only seen in input samples whereas the pulldown and controls show no bumps at all.Even the tei meth histone L27 pulldown used as positive control fails to show peaks. where as the PCR verification of targets for controls works fine.
I am using HS DNA kit for BA. Magnify kit for pulldown. The kit elutes the DNA finally in 150 ul. which is too dilute to work with. has anyone else worked with this kit and what is the next step to say doing a NGS chip seq.
Thanks
These are only seen in input samples whereas the pulldown and controls show no bumps at all.Even the tei meth histone L27 pulldown used as positive control fails to show peaks. where as the PCR verification of targets for controls works fine.
I am using HS DNA kit for BA. Magnify kit for pulldown. The kit elutes the DNA finally in 150 ul. which is too dilute to work with. has anyone else worked with this kit and what is the next step to say doing a NGS chip seq.
Thanks
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