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  • Experimental Design - Single Cell RNA-Sequencing

    Hi,

    I am designing scRNA-Seq experiment on two cancer cell lines grown in specific conditions. I am curious as to what can be considered control in such experiments. In case of primary tumors, one can take adjacent healthy cells as controls.

    Also, is a control really required in such a experiment where a few thousand cells are being analysed to find different sub populations. I have been referencing some literature and there seems to be no consensus.

    Please share your views.

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