Hi,
Has anyone got a method for assessing TruSeq ligation efficiency, pre-PCR? We use the KAPA Illumina library quant kit for final library quantification, and I can certainly try using this, but I am wondering if anyone knows about actually using the results to assess the efficiency? I'm not sure how to evaluate the results to determine next steps.
Thanks in advance!
Has anyone got a method for assessing TruSeq ligation efficiency, pre-PCR? We use the KAPA Illumina library quant kit for final library quantification, and I can certainly try using this, but I am wondering if anyone knows about actually using the results to assess the efficiency? I'm not sure how to evaluate the results to determine next steps.
Thanks in advance!
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