sequencing short fragments of single strand DNA

Fahma · 07-30-2019, 12:17 AM

Hello every body

I have a solution of short fragments of single strand DNA between 100-200 bases.
How can I sequence them?

Thank you.

nucacidhunter · 07-30-2019, 03:32 AM

You can use ssDNA library prep method or a kit such as ACCEL-NGS 1S (or
1S Plus) DNA LIBRARY kit.

BioDynami · 07-30-2019, 06:48 PM

NGS Single Stranded DNA Library Prep Kit:

Kit advantages:

• Fast
- Total time: 1.5 hrs
- Hands-on time: 10 min
• Simple workflow: Less steps
• Guaranteed quality: High library conversion efficiency
• Single stranded DNA as input: From 50 ng to 500 ng

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07-30-2019, 12:17 AM	#1
Fahma Junior Member Location: ferman Join Date: Jul 2019 Posts: 1	sequencing short fragments of single strand DNA Hello every body I have a solution of short fragments of single strand DNA between 100-200 bases. How can I sequence them? Thank you.

07-30-2019, 06:48 PM	#3
BioDynami Registered Vendor Location: Alabama Join Date: Jul 2016 Posts: 23	NGS Single Stranded DNA Library Prep Kit: Kit advantages: • Fast - Total time: 1.5 hrs - Hands-on time: 10 min • Simple workflow: Less steps • Guaranteed quality: High library conversion efficiency • Single stranded DNA as input: From 50 ng to 500 ng

07-30-2019, 03:32 AM	#2
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	You can use ssDNA library prep method or a kit such as ACCEL-NGS 1S (or 1S Plus) DNA LIBRARY kit.