Hey everyone. I'm very new to next gen sequencing and will be planning on using a MiSeq in the near future. I had a question about number of reads. If we are looking at targeted gene expression, the number of reads required depends on how many genes we are targeting correct? So if we were to target around 200 genes what would be the calculation for finding out how many reads would be sufficient for each gene?
Here's my thoughts and calculations using Illumina's Reagent kit V3:
(25 million reads)/(200 targeted genes)] = 125,000 reads/gene
(125,000 reads/gene)/(50 samples) = 2,500 reads/(gene*sample)
Would 2,500 reads per gene in each sample be overkill? Would increasing the number of samples instead be more beneficial? Thanks for all the help and I apologize in advance if this is basic stuff.
Here's my thoughts and calculations using Illumina's Reagent kit V3:
(25 million reads)/(200 targeted genes)] = 125,000 reads/gene
(125,000 reads/gene)/(50 samples) = 2,500 reads/(gene*sample)
Would 2,500 reads per gene in each sample be overkill? Would increasing the number of samples instead be more beneficial? Thanks for all the help and I apologize in advance if this is basic stuff.
Comment