Can anyone let me know if I have this right?
For the Illumina ChIP kit library preparation, there is a size selection step following adapter ligation for fragments +/- 25bp around 200bp.
The adapter sequences for genomic library prep are 92nt (from another thread on this forum).
So this means that the only fragments included in the library will be 108bp +/-25.
With sonication or enzymatic fragmentation of DNA, most people seem to acheive a mean fragment size of around 150-300bp.
Wouldn't this make a less than ideal library??
For the Illumina ChIP kit library preparation, there is a size selection step following adapter ligation for fragments +/- 25bp around 200bp.
The adapter sequences for genomic library prep are 92nt (from another thread on this forum).
So this means that the only fragments included in the library will be 108bp +/-25.
With sonication or enzymatic fragmentation of DNA, most people seem to acheive a mean fragment size of around 150-300bp.
Wouldn't this make a less than ideal library??
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