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  • Parsing position in a protein sequence using perl

    Hi!

    I'd like to ask for help because I'm trying to deduce the relative positions in an ungapped sequence given the positions in a gapped sequence (from a multiple sequence alignment).
    In order to simplify my task I substituted aminoacids with capital "A"s and gaps with "0"s.


    I'm trying to do this using Perl, but my code isn't behaving correctly (and I can't figure out why).

    So, here's my issue. I have to deduce positions in a "simple string" given positions in a "complete string".
    Here's a minimal exemple of my input file:
    Code:
    #name   complete(cs)    len(cs) simple(ss)  len(ss) pos(cs)
    NAME1   A0AAA000AAA00A  14      AAAAAAAA    8        4,6            
    NAME2   AAAA0AA00000A   13      AAAAAAA     7        7
    Here's my code:
    Code:
    $ perl -anle '
        print "$_ position(cs)" and next if /^#/;
        printf "%s",$_;
        for $pos_ss (split ",",$F[5]) {
            $char = substr($F[1],$pos_ss-1,1);
            @cs = split //,$F[3];
            @cs_idx = grep {$cs[$_] eq $char} 0..$#cs;
            push @res,++$cs_idx[$pos_ss-1];
        }
        printf "%14s\n", join ",",@res;
        @res=();
    ' file
    And here's my expected output:
    Code:
    NAME1   A0AAA000AAA00A  14      AAAAAAAA    8        4,9	3,5
    NAME2   AAAA0AA00000A   13      AAAAAAA     7        7          6
    In the provided exemple, I have to say that the 4th character (which is the 3rd "A") in the complete string(cs) corresponds to the the 3rd character in the simple string(ss), and so on...

    Could you please help me formatting/reviewing my code?

    Thanks in advance for your precious help!!

    EDIT
    Non-coding help is also greatly accepted! ie: how would you proceed if you had to do this job?
    Last edited by dovah; 07-31-2014, 05:12 AM.

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