When I did 454 I just amplified my gene using fusion primers that had the gsp, then the barcode then the adaptor primer all in one oligo (barcoded forward and reverse)--and I just had IDT or whoever synthesize the oligo. I cannot seem to find out if this is doable with Illumina library prep. What is the easiest way to barcode your amplicon and prep it for Illumina sequencing? All of the instructins I have foudn seem so complicated compared to 454!
Please help, thanks
John
Please help, thanks
John
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