I ran a HiSeq on environmental samples and the purpose of the run was to blast my sequences against the NCBI-nr database to see what species my reads match to. I am not doing a denovo assembly or genome assembly.
My FastQc report passes in all aspects except: per base sequence content, per sequence GC content and kmer content.
Should I be worried? How much should I rely on a fastqc report?
Thank you in advance!
My FastQc report passes in all aspects except: per base sequence content, per sequence GC content and kmer content.
Should I be worried? How much should I rely on a fastqc report?
Thank you in advance!
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