Tweet
Suggestions for thermal cyclers that work with Pico PCR plates
Dear all,
I am about to start a new set of single-cell RNA sequencing experiments, and I am trying to get the basics right. I have a very rare cell population (10:ths to 100:ths of cells in a sample) and I am limited to 10 minutes sorting time per plate. This means that I cannot possibly sort more than 96 wells before having to freeze the plate. As I will run a considerable number of samples, I would however like to run the PCRs etc in 384 format. For this reason, I was very happy to find the Pico PCR plates from Thermo Fisher, https://www.thermofisher.com/order/c...duct/SPL096050, that have been used in some papers cited a lot in this forum, such as https://www.ncbi.nlm.nih.gov/pubmed/26878113 . But when I contact Thermo, they first of all tell me that they ave discontinued the Pico thermal cyclers that were created to run these Pico plates, and furthermore, they cannot provide me with a list of machines that would work with four of the Pico 96 well plates snapped into a plate frame. If anyone could advice me on how they solve this problem, I would be very thankful. As I see it, I either ask for suggestions for a thermal cycler that can take these plates in their frames, that will be like a skirted 384 plate, or for suggestions to completely different approaches to the problem. Thank you very much in advance!
Best regards
Jakob
I am about to start a new set of single-cell RNA sequencing experiments, and I am trying to get the basics right. I have a very rare cell population (10:ths to 100:ths of cells in a sample) and I am limited to 10 minutes sorting time per plate. This means that I cannot possibly sort more than 96 wells before having to freeze the plate. As I will run a considerable number of samples, I would however like to run the PCRs etc in 384 format. For this reason, I was very happy to find the Pico PCR plates from Thermo Fisher, https://www.thermofisher.com/order/c...duct/SPL096050, that have been used in some papers cited a lot in this forum, such as https://www.ncbi.nlm.nih.gov/pubmed/26878113 . But when I contact Thermo, they first of all tell me that they ave discontinued the Pico thermal cyclers that were created to run these Pico plates, and furthermore, they cannot provide me with a list of machines that would work with four of the Pico 96 well plates snapped into a plate frame. If anyone could advice me on how they solve this problem, I would be very thankful. As I see it, I either ask for suggestions for a thermal cycler that can take these plates in their frames, that will be like a skirted 384 plate, or for suggestions to completely different approaches to the problem. Thank you very much in advance!
Best regards
Jakob