Does anyone know the sequences of the PCR enrichment primers from the Illumina TruSeq kit (the PPC primers)? I have the adaptor sequences, so I know what the 5' end of each sequence should be, but I don't know how long they are.
Additional info:
I tried the sequences from the OpenWetWare protocol in this thread:
They are as follows:
P5_PCR: AATGATACGGCGACCACCGAG
P7_PCR: CAAGCAGAAGACGGCATACGAG
These do amplify the library, but they are very inefficient compared to the PPC primers in the kit.
I have seen other examples of primers in the literature that are longer. However, the longer ones that I have seen include the region of P7 where the index is. I hope I do not need to make a separate P7 primer for each index in order to preserve my ability to multiplex. I'm sure that is not how the PPC primers work.
Thanks!
Sean
Additional info:
I tried the sequences from the OpenWetWare protocol in this thread:
They are as follows:
P5_PCR: AATGATACGGCGACCACCGAG
P7_PCR: CAAGCAGAAGACGGCATACGAG
These do amplify the library, but they are very inefficient compared to the PPC primers in the kit.
I have seen other examples of primers in the literature that are longer. However, the longer ones that I have seen include the region of P7 where the index is. I hope I do not need to make a separate P7 primer for each index in order to preserve my ability to multiplex. I'm sure that is not how the PPC primers work.
Thanks!
Sean
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