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Old 07-23-2020, 12:31 PM   #1
kluttgeharm
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Location: Iowa

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Default Sequencing through the insert

Hi I am wondering how common it is for libraries to sequence through the adapter and run out of nucleotide to sequence like in this Illumina video. (https://www.youtube.com/watch?v=bkMiy5lGIqs)

Is it recommended to analyze the size of you library prior to deciding on read lengths?

Last edited by kluttgeharm; 07-24-2020 at 06:06 AM.
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Old 07-23-2020, 05:15 PM   #2
luc
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Yes, you definitely want to QC your library for the fragment lengths before sequencing using a Bioanalyzer or equivalent devices.
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