Hi,
I have trancriptome file consisting around 39000 DNA sequences. Now I would like find the longest ORFs for all the 39000 DNA sequences. I used EMBOSS's getORF from its webservice to find the all the possible ORFs. As I kept the default parameter 30 for minimum number of amino acids for a peptide, I got lot of ORFs which has more than 30 amino acids sequences for a single transcript. Now I would like to retain only the longest peptide with maximum of number of amino acids for all sequences.
How can I achieve that? is there any alternate way to get only the longest ORF fro all transcript? Kindly guide me
I have trancriptome file consisting around 39000 DNA sequences. Now I would like find the longest ORFs for all the 39000 DNA sequences. I used EMBOSS's getORF from its webservice to find the all the possible ORFs. As I kept the default parameter 30 for minimum number of amino acids for a peptide, I got lot of ORFs which has more than 30 amino acids sequences for a single transcript. Now I would like to retain only the longest peptide with maximum of number of amino acids for all sequences.
How can I achieve that? is there any alternate way to get only the longest ORF fro all transcript? Kindly guide me
Comment