Hi everyone
Probably a stupid question, but i'm finding it hard to find meaningful info on sequencing depth and read numbers on the MiSeq.
Illumina states that a 16S V4 run should deliver ~20m reads.
Assuming 96 samples are run, that's 208,333 raw reads per sample.
Therefore, if I run 24 samples, should I expect 833,333 raw reads per sample?
I guess my question is why does the number of sequences fall with increased samples?
Thank you
Probably a stupid question, but i'm finding it hard to find meaningful info on sequencing depth and read numbers on the MiSeq.
Illumina states that a 16S V4 run should deliver ~20m reads.
Assuming 96 samples are run, that's 208,333 raw reads per sample.
Therefore, if I run 24 samples, should I expect 833,333 raw reads per sample?
I guess my question is why does the number of sequences fall with increased samples?
Thank you
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