I apologize if this has been hashed out already, but I cannot seem to find any definitive documentation which describes whether the flowcell oligos are P5 and P7, or the complements. I know Illumina considers this information proprietary so it's not immediately obvious to me. I am wondering whether or not an excess of free P5 and P7 custom PCR primer (not dimer) would bind to the flowcell. I understand why dimers (which amplify and thus contain both P5/complement to P5, etc) would bind to the flowcell, but what about just these oligos?
My primers are:
SLXP5_PCR - AATGATACGGCGACCACCGAG
SLXP7_PCR - CAAGCAGAAGACGGCATACGAG
Anybody have a good, clear resource on this?
Thanks in advance!
My primers are:
SLXP5_PCR - AATGATACGGCGACCACCGAG
SLXP7_PCR - CAAGCAGAAGACGGCATACGAG
Anybody have a good, clear resource on this?
Thanks in advance!
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