Hey guys,
I want to perform RNA-seq on FACS sorted material. That means I will have some degradation and low input (around 10 ng and potentially less). Also I want to have quantitative data for differential expression analysis why I think poly-A selection is the method of choice. Does anybody have experience or literature on which preparations kits work best? Or a strong opinion about why total RNA might also work well using some particular kit?
(We are talking about Drosophila here).
That would be great
Cheers
M. S.
I want to perform RNA-seq on FACS sorted material. That means I will have some degradation and low input (around 10 ng and potentially less). Also I want to have quantitative data for differential expression analysis why I think poly-A selection is the method of choice. Does anybody have experience or literature on which preparations kits work best? Or a strong opinion about why total RNA might also work well using some particular kit?
(We are talking about Drosophila here).
That would be great
Cheers
M. S.
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