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Old 09-16-2020, 07:56 AM   #1
ecSeq Bioinformatics
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Location: Leipzig, Germany

Join Date: May 2012
Posts: 268
Lightbulb DNA Methylation Data Analysis Workshop - 2-5 November 2020 in Berlin



How to use bisulfite-treated sequencing to study DNA methylation
Advance your epigenetics research with NGS methods.

Link to workshop website

In a nutshell
  • Learn how bisulfite sequencing works
  • Understand how bisulfite-treated reads are mapped to a reference genome
  • Perform basic analyses (call methylated regions, perform basic downstream analyses)
  • Use shell scripting to create reusable data pipelines
  • Visualize results (ready-to-publish)

The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:
  • be familiar with the sequencing method of Illumina
  • understand how bisulfite sequencing works
  • be aware of the mapping problem of bisulfite-treated data
  • understand how bisulfite-treated reads are mapped to a reference genome
  • be familiar with common data formats and standards
  • know relevant tools for data processing
  • automate tasks with shell scripting to create reusable data pipelines
  • perform basic analyses (call methylated regions, perform basic downstream analyses)
  • plot and visualize results (ready-to-publish)
  • be able to reuse all analyses

More information here
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