Hi,
I have just started shearing my DNA for downstream exome library preparation.
I used the illumina recommended protocol on the Covaris M220 instrument, aiming at peak top at ~250 bp.
Then I checked the samples on High Sensitivity chip(bioanalyzer) and got the results as in the attachment.
Of what I have seen in other threads it looks generally ok, but I puzzled about the shift in the "graph shoulder" on some samples?
Any comment on the results in general and on the issue I pointed out will be appreciated.
Ragards,
see
I have just started shearing my DNA for downstream exome library preparation.
I used the illumina recommended protocol on the Covaris M220 instrument, aiming at peak top at ~250 bp.
Then I checked the samples on High Sensitivity chip(bioanalyzer) and got the results as in the attachment.
Of what I have seen in other threads it looks generally ok, but I puzzled about the shift in the "graph shoulder" on some samples?
Any comment on the results in general and on the issue I pointed out will be appreciated.
Ragards,
see
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