Hi Everyone,
Just a quick question.
I'm currently trying to enrich a library prep using solution hybrid capture. I'm following the Nextera Enrichment protocol however because i'm studying bacteria I cannot use the kit itself.
I'm just wondering if anyone knows what the buffers ET1 and HP3 contain? They are used to elute samples from a streptavidin/biotin complex. I'm guessing they denature the DNA so its released from the biotinylated primer.
If not does anyone have a suggestion that would be fit to use before sequencing on a MiSeq.
Adam
Just a quick question.
I'm currently trying to enrich a library prep using solution hybrid capture. I'm following the Nextera Enrichment protocol however because i'm studying bacteria I cannot use the kit itself.
I'm just wondering if anyone knows what the buffers ET1 and HP3 contain? They are used to elute samples from a streptavidin/biotin complex. I'm guessing they denature the DNA so its released from the biotinylated primer.
If not does anyone have a suggestion that would be fit to use before sequencing on a MiSeq.
Adam
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