Go Back   SEQanswers > Core Facilities

Similar Threads
Thread Thread Starter Forum Replies Last Post
300 Stool Samples - 16s rDNA amplification & sequencing for identifying bacteria vs92 454 Pyrosequencing 5 11-20-2013 08:28 AM
How to detect Heterozygous (double) peaks from Sanger shuang Bioinformatics 10 01-25-2013 12:22 PM
Strange peaks in TruSeq RNA library TonyBrooks Sample Prep / Library Generation 5 08-26-2012 02:24 AM
SHRiMP and Cufflinks for strand-specific SOLiD bacteria RNA Seq questions pjorth Bioinformatics 0 05-17-2011 12:45 PM
RNA-Seq: Deep sequencing-based transcriptome profiling analysis of bacteria-challenge Newsbot! Literature Watch 0 08-17-2010 02:00 AM

Thread Tools
Old 09-28-2012, 12:43 AM   #1
Junior Member
Location: Colombo, Sri Lanka

Join Date: Aug 2012
Posts: 5
Default Heterozygous peaks in bacteria RNA sequencing

hello all,

When I try to sequence 16s bacterial RNA using ABI 3130 it gives me heterozygous peaks. Since the microbes only contain a haploid set of chromosomes I am puzzled how it could be possible to indicate two peaks?
can someone please explain
Sujani is offline   Reply With Quote
Old 09-28-2012, 07:44 AM   #2
Senior Member
Location: USA, Midwest

Join Date: May 2008
Posts: 1,165

Because bacteria typically have multiple copies of the 16S rRNA gene?
kmcarr is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:46 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO