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Hi all, while looking for SNPs for virus quasispecies in a badnavirus using RNA that was gel-excised with maximum length of 100, maybe 200 bp I find definitive read alignment hotspots to the master genome in all 3 samples. generally people have been finding these hotspots in the populations of siRNA of 21,22 or 24 nt length, products of silencing. however the hotspots I find are in the slightly longer reads. Sequencing ran for 36 cycles, so they are 36 nt long. Also, 90+% of these reads with potential lengths between 30 and 100 nt are aligning to the sense strand. I am trying to come up with an explanation for these two things and drawing a blank. I am not a virologist or even microbiologist, so am I missing something obvious? I apologize if this is not the place to ask this question, and would appreciate any answers or other places to ask this question.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM