Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Next-generation DNA sequencing-based assay for measuring allelic expression i Newsbot! Literature Watch 0 10-23-2011 02:40 AM
PubMed: Droplet-Based Pyrosequencing Using Digital Microfluidics. Newsbot! Literature Watch 0 09-22-2011 07:40 AM
PubMed: Genomic DNA pooling strategy for next-generation sequencing-based rare varian Newsbot! Literature Watch 0 09-18-2011 08:30 AM
PubMed: Sequencing nucleic acids: from chemistry to medicine. Newsbot! Literature Watch 0 05-06-2011 03:50 AM
PubMed: Picoliter DNA sequencing chemistry on an electrowetting-based digital microfl Newsbot! Literature Watch 0 12-21-2010 06:50 AM

Thread Tools
Old 02-08-2011, 02:00 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Picoliter DNA sequencing chemistry on an electrowetting-based digital microfl

Syndicated from PubMed RSS Feeds

Picoliter DNA sequencing chemistry on an electrowetting-based digital microfluidic platform.

Biotechnol J. 2011 Feb;6(2):165-76

Authors: Welch ER, Lin YY, Madison A, Fair RB

The results of investigations into performing DNA sequencing chemistry on a picoliter-scale electrowetting digital microfluidic platform are reported. Pyrosequencing utilizes pyrophosphate produced during nucleotide base addition to initiate a process ending with detection through a chemiluminescence reaction using firefly luciferase. The intensity of light produced during the reaction can be quantified to determine the number of bases added to the DNA strand. The logic-based control and discrete fluid droplets of a digital microfluidic device lend themselves well to the pyrosequencing process. Bead-bound DNA is magnetically held in a single location, and wash or reagent droplets added or split from it to circumvent product dilution. Here we discuss the dispensing, control, and magnetic manipulation of the paramagnetic beads used to hold target DNA. We also demonstrate and characterize the picoliter-scale reaction of luciferase with adenosine triphosphate to represent the detection steps of pyrosequencing and all necessary alterations for working on this scale.

PMID: 21298802 [PubMed - in process]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:30 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO