Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
Size selection for Nextera libraries? sweetph3 Sample Prep / Library Generation 9 08-18-2015 01:50 AM
Purification of Nextera libraries for use on the MiSeq vieiraL General 5 03-27-2013 04:26 AM
Genomic DNA libraries using Nextera creeves Illumina/Solexa 3 03-02-2013 11:03 AM
qPCR on Nextera XT Libraries kcchan Sample Prep / Library Generation 3 08-30-2012 07:29 AM
Concentrating NexteraXT libraries docbio Sample Prep / Library Generation 4 08-24-2012 10:11 AM

Thread Tools
Old 02-07-2019, 08:35 PM   #1
Junior Member
Location: australia

Join Date: Feb 2019
Posts: 2
Smile concentrating Nextera XT libraries

Hi, We need to concentrate Nextera XT DNA libraries before baiting with MyBaits. The libraries are 22ul and total DNA ranges from 130-160ng. The minimum recommended input DNA for baiting is 100ng and volume is 7ul. Do others have experience of best way to concentrate Speedvac? MinElute? Or something else. These are clinical samples and there is a very limited amount of DNA left.
Extraordinarily grateful for any/all advice offered
CatWoman is offline   Reply With Quote
Old 02-07-2019, 10:27 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,220

If libraries are eluted in water, SpeedyVac is the best option otherwise Zymo DNA Clean & Concentrator (DCC-5) which allows elution with minimum 6 ul.
nucacidhunter is offline   Reply With Quote
Old 02-09-2019, 04:01 PM   #3
Junior Member
Location: australia

Join Date: Feb 2019
Posts: 2
Default concentrating Nextera XT libraries

Thank you very much for your help
CatWoman is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:45 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO