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Has anyone done suppression subtractive hybridization PCR (SSH) on cDNA prior to 454 sequencing? Any tips, protocols, etc would be greatly appreciated.
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Probably not what you are looking for, but the following uses an Evrogen kit to normalize cDNA libraries prior to 454 sequencing:Originally posted by AKroxy View Post
http://www.pubmedcentral.nih.gov/art...?artid=2689275
Evrogen also sells SSH reagents, so that could be a place to look.
Phillip -
ssh + 454
Hello,
Do you know if are there some information about supression substrative hubridization using clontech kit prior to pyrosequencing 454???
Thanks in advance!!!Comment
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I tried tweaking that protocol to get rid of homopolymers so we could use the samples in 454 sequencing, but I failed. I would have needed to spend a lot more time on it to get it to work.Comment
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