Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    GSEA on Cuffdiff log2(fold_change) values

    Hi:

    I am using Cuffdiff log2(fold_change) values in 'rnk' file for GSEA.

    Althought I had 8K genes, my C2_all set enrichment was only 1 gene set at FDR-25%.

    My question is - is log2(fold_change) values - suitable for GSEA enrichment.

    I mean - can I compare the AFfy log2 fold changes and cuffdiff log2(fold_change) values and do Pre-ranked enrichment.

    Is the distribution meaningful for GSEA enrichment.

    thanks
    Adrian.
    Tags: None
Previous template Next

Latest Articles

Collapse
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
Cancer Metastasis: A Deep Dive into Cellular Plasticity by seqadmin
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
55 views
0 likes
 Last Post seqadmin
by seqadmin
04-11-2024, 12:08 PM  
Proteogenomic Profiles Offer New Clues in Prostate Cancer by seqadmin
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
52 views
0 likes
 Last Post seqadmin
by seqadmin
04-10-2024, 10:19 PM  
Novel Diagnostic Assay Enhances Ovarian Cancer Detection by seqadmin
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
45 views
0 likes
 Last Post seqadmin
by seqadmin
04-10-2024, 09:21 AM  
Evolutionary Dynamics of Centromeres: A Comparative Genomic Analysis by seqadmin
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
 Last Post seqadmin
by seqadmin
04-04-2024, 09:00 AM  
View All
Working...
X