Hi,
I extracted the DNA by Chloroform extraction and had also a very high yield. But unfortunately as I started sequencing with the PacBio platform I had a veryy low number of reads. I assume that some organic molecules interfere with the polymerase. My bacterial strain is a strong EPS Producer and this is maybe the cause for the low read numbers.
Does anybody have any experience whith such Problems? Or can recommend me a protocol where I certainly get rid of all the EPS?
Thanks a lot...
Limno
I extracted the DNA by Chloroform extraction and had also a very high yield. But unfortunately as I started sequencing with the PacBio platform I had a veryy low number of reads. I assume that some organic molecules interfere with the polymerase. My bacterial strain is a strong EPS Producer and this is maybe the cause for the low read numbers.
Does anybody have any experience whith such Problems? Or can recommend me a protocol where I certainly get rid of all the EPS?
Thanks a lot...
Limno
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