Hi,
I've sequenced (Illumina GA II) 6 leukemic samples and a sample composed of a pool of 3 control. The purpose of the work is the analysis of the miRNAome and the up/down regulated miRNA.
For the DE analysis, I use the DESeq package.
So I've a matrix where each colum is a sample and each line a miRNA.
Since my control is a pool of 3 control sample, can I use three same columns for the control. Example :
Three same columns
or
Anyone can help me ?
Thanks,
N.
I've sequenced (Illumina GA II) 6 leukemic samples and a sample composed of a pool of 3 control. The purpose of the work is the analysis of the miRNAome and the up/down regulated miRNA.
For the DE analysis, I use the DESeq package.
So I've a matrix where each colum is a sample and each line a miRNA.
Since my control is a pool of 3 control sample, can I use three same columns for the control. Example :
Three same columns
Code:
leu1 leu2 leu3 ctrl ctrl' ctrl'' miR-1 x y z a a a miR-2 x' y' z' a' a' a' . .
Code:
One colum for the pool of 3 ctrl leu1 leu2 leu3 ctrl miR-1 x y z a miR-2 x' y' z' a' . .
Anyone can help me ?
Thanks,
N.
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