Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
Overclustered sequencing (low PF, low Q30) V2 2x250 hcarrington Illumina/Solexa 5 07-07-2015 10:43 AM
RNA shearing Staroselec RNA Sequencing 4 07-02-2014 06:49 AM
Wang, 'Low Cost Library Construction...': Low Concentration MAdkisson RNA Sequencing 2 02-19-2014 02:33 PM
EDTA interferes with library prep? Nilorac RNA Sequencing 1 04-18-2012 08:50 AM
DNA shearing suludana Illumina/Solexa 18 03-01-2010 06:30 PM

Thread Tools
Old 02-15-2016, 04:02 AM   #1
Junior Member
Location: Germany

Join Date: Jul 2011
Posts: 6
Default Difference when shearing with Low-EDTA and Water?

Dear all,
Does anyone have ever test the shearing protocol provided by Covaris with different buffer? e.g. low-EDTA buffer and Water?
thank you!
wangwde is offline   Reply With Quote
Old 02-15-2016, 05:05 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,235

TE buffer which has been recommended is the best option. For fragmentation to small sizes 250bp< degradation level increases if water is used for resuspension of DNA.
nucacidhunter is offline   Reply With Quote
Old 02-17-2016, 03:36 PM   #3
Junior Member
Location: Arizona

Join Date: Nov 2015
Posts: 2

I haven't compared all different buffer types but we routinely use TE - low EDTA (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) with excellent results, fragmenting to ~160bp.
neurula is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:55 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO