Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
NuGen Ovation Universal RNA-seq on Torrent zara Ion Torrent 2 01-27-2015 12:59 AM
NuGEN Ovation® RNA‑Seq with PacBio sequencing aliceli Sample Prep / Library Generation 0 11-28-2012 06:52 AM
New! Ovation RNA-Seq System V2 from NuGEN Technologies NuGEN Vendor Forum 2 07-21-2011 08:46 PM
Alternatives to Nugen Ovation RNA-Seq pbluescript Sample Prep / Library Generation 1 02-13-2011 01:14 PM
Experience with nugen ovation 3 dge System moritzhess Sample Prep / Library Generation 0 11-06-2010 01:38 PM

Thread Tools
Old 11-18-2019, 01:04 AM   #1
Junior Member
Location: Germany

Join Date: Nov 2019
Posts: 1
Default Mixing cDNA samples from Ovation RNA‐Seq System V2 of NuGEN with Nextera Dual Indexes


I have a question regarding the compatibility of the "IDT® for Illumina Nextera™ DNA UD Indexes". Illumina says "These index kits are NOT interchangeable with the Nextera DNA or Nextera XT Index Kits".
But, are they compatible with amplified cDNA samples prepared using the Ovation® RNA‐Seq System V2 of NuGEN from RNA?
Our preferred sequence facility (uses HiSeq4000) has strongly recommended the use of duel index adaptors (in specific the Ovation® Ultralow System V2 developed by Illumina) to avoid index hoping, we will like to know if Nextera indexes are also an option,

Best regards
seq_uestion is offline   Reply With Quote
Old 11-19-2019, 07:34 AM   #2
Senior Member
Location: US

Join Date: Dec 2010
Posts: 458

Best ask Nugen. Likely Nugen is using the Truseq design for their kits, not Nextera sequences.
luc is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:18 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO