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Old 04-02-2014, 09:54 PM   #1
woodydon
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Default Would RNA quality/quantity be good enough for sequencing when the liver tumor samples

Dear all,

Would RNA quality/quantity be good enough for sequencing when the liver tumor samples are frozen and sliced?

One of my colleague thinks that the RNA quality should be fine for those cryo-sliced liver tumor samples. We just need to use more sliced samples to get enough RNA. Is this correct?

One of my friends told me that the sliced liver tumor samples will have poor RNA quality, so I am worried. I am a totally dry lab geek. Could please someone help me? Thanks!

Woody

Last edited by woodydon; 04-02-2014 at 09:59 PM.
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Old 04-03-2014, 12:39 AM   #2
dpryan
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The only way to find out would be to extract RNA from a bunch of slices and have a look on a bioanalyzer. I've sequenced RNA from frozen liver samples without issue, so unless the cryo-slicing wasn't really cryo enough then I would expect it'd work.
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Old 04-03-2014, 04:34 AM   #3
woodydon
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Quote:
Originally Posted by dpryan View Post
The only way to find out would be to extract RNA from a bunch of slices and have a look on a bioanalyzer. I've sequenced RNA from frozen liver samples without issue, so unless the cryo-slicing wasn't really cryo enough then I would expect it'd work.
Hi dpryan,

Thanks!

Woody
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