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Old 02-15-2016, 04:02 AM   #1
wangwde
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Location: Germany

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Default Difference when shearing with Low-EDTA and Water?

Dear all,
Does anyone have ever test the shearing protocol provided by Covaris with different buffer? e.g. low-EDTA buffer and Water?
thank you!
Wei
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Old 02-15-2016, 05:05 PM   #2
nucacidhunter
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TE buffer which has been recommended is the best option. For fragmentation to small sizes 250bp< degradation level increases if water is used for resuspension of DNA.
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Old 02-17-2016, 03:36 PM   #3
neurula
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I haven't compared all different buffer types but we routinely use TE - low EDTA (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) with excellent results, fragmenting to ~160bp.
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