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Hi all,
Last month, I made fixed tissue (5< RIN < 7) RNA-seq library using SMART-seq2 method. (I thought single cell library prep method is suitable for fixed sample because of low quantity of RNA and even broken RNA could be captured by poly-A primer in RT )
However, I found that multiple TSO, ISPCR sequences were included in library reads(Mostly less than 300bp reads). Even though I checked library quality by DNA high sensitivity chip, but almost 50% of reads has mostly TSO oligomers.
Is there anyone who faced same problems? How can I solve this? Any suggestion would be welcomed
Last month, I made fixed tissue (5< RIN < 7) RNA-seq library using SMART-seq2 method. (I thought single cell library prep method is suitable for fixed sample because of low quantity of RNA and even broken RNA could be captured by poly-A primer in RT )
However, I found that multiple TSO, ISPCR sequences were included in library reads(Mostly less than 300bp reads). Even though I checked library quality by DNA high sensitivity chip, but almost 50% of reads has mostly TSO oligomers.
Is there anyone who faced same problems? How can I solve this? Any suggestion would be welcomed
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