Hi everyone, i'm trying to run de novo assembly using velvet on short (100bp long) paired end reads.
I first ran velveth and it created only two files named: Log and Sequences.
It also gave me this error:
velveth: Can't malloc 121 chars: Cannot allocate memory
I tried to run velvetg but then I got an error:
[0.000000] No Roadmap file to build upon! Please run velveth (see manual)
This is what I ran using velveth:
$ velveth output_assem 35 -shortPaired -fasta file_PE-1.fa file_PE-2.fa
The two fasta files are already in paired
What does this mean? This is my first time using velvet, and I will appreciate anyone's help. Thanks a lot in advance
I first ran velveth and it created only two files named: Log and Sequences.
It also gave me this error:
velveth: Can't malloc 121 chars: Cannot allocate memory
I tried to run velvetg but then I got an error:
[0.000000] No Roadmap file to build upon! Please run velveth (see manual)
This is what I ran using velveth:
$ velveth output_assem 35 -shortPaired -fasta file_PE-1.fa file_PE-2.fa
The two fasta files are already in paired
What does this mean? This is my first time using velvet, and I will appreciate anyone's help. Thanks a lot in advance
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