Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
Can Chromasig be used to analyse multiple DNA methylation samples? blackjimmy Epigenetics 1 07-20-2014 12:48 AM
Degradation of DNA Samples megalodon Sample Prep / Library Generation 6 11-16-2011 05:51 AM
PubMed: Efficient storage of high throughput DNA sequencing data using reference-base Newsbot! Literature Watch 0 09-07-2011 02:00 AM
Shipping DNA samples from Europe to U.S. PRJ321 General 4 08-02-2011 02:57 AM
Can I use sheared genomic DNA as starting material of bacterial genome sequencing? omnivore 454 Pyrosequencing 6 10-13-2010 05:11 AM

Thread Tools
Old 08-01-2011, 12:50 AM   #1
Junior Member
Location: singapore

Join Date: Jul 2011
Posts: 3
Default storage of sheared DNA samples overnight


I am a new user with Covaris. I build DNA sequence libraries from clinical samples. I extract DNA from the samples and shear them to sizes 150 - 200bp and use these fragments to build sequencing libraries (using illumina multiplex sequencing kit). After shearing on covaris, is it advisable to store the fragments overnight and build library the next day? If it is alright, are there any precautions or things to take note of in storing them?
lizhxp is offline   Reply With Quote
Old 08-01-2011, 08:17 AM   #2
Senior Member
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317

I don't foresee any issues with doing this other than the obvious ones. For example, if your DNA is not purified of contaminating DNAses prior to sonication, then it may degrade while being left overnight. But, if you are not seeing any issues with degradation prior to sonication, you are probably fine.

pmiguel is offline   Reply With Quote
Old 08-01-2011, 09:14 AM   #3

Posts: n/a

I have done this as well. It's probably not the best practice for sheared DNA, but I have not personally seen catastrophic degradation.
  Reply With Quote
Old 08-04-2011, 07:25 AM   #4
Junior Member
Location: US

Join Date: Jan 2011
Posts: 6

Overnight at 4 degrees should not be a problem.
n2onme is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:34 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO