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Old 09-20-2013, 09:42 AM   #1
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Location: San Diego

Join Date: Sep 2013
Posts: 4
Default TruSeq and NEBNext RNA primer dimers around 100bp

I have been prepping libraries with both the NEBNext Ultra RNA kit for Illumina Seq and the TruSeq RNA. There is an abnormally high concentration of primer dimers around 100bp, especially with the NEBNext kit. Has anyone encountered this problem or know how to fix it. I am not sure why the AMPure bead cleanup is not getting rid of these fragments either. Any help would be greatly appreciated.
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