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Old 01-04-2013, 11:53 AM   #1
ChIP Kick
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Location: Phoenix

Join Date: Dec 2012
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Default ChIP reagents

Hi, I tried many times but I still cannot get a good sonication condition for my exp. Now I used all my lysis buffer which I bought from Millipore(EZ-Magna ChIPô A Chromatin Immunoprecipitation Kit). I checked their web but no recipe for it.There are so many different recipes for lysis buffer. I do not know which one is good for my exp.

So is there someone who could give me suggestions to make my own cell lysis buffer and nuclear lysis buffer?

Thanks a lot!
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Old 01-07-2013, 06:37 AM   #2
kwaraska
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Do you have access to any Coveris Instrument? They have a very good ChIP kit which has worked well for most of my customers.

I would suggest contacting Covaris (www.covarisinc.com) and finding out if they have an instrument in the area....

Unless of course you are in Boston-then you can use ours.
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Old 01-07-2013, 09:49 PM   #3
ChIP Kick
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Thanks! Unfortunately we don't have Coveris Instrument. I can fly to Boston to use yours...I am just kidding

I still need to make my own cell lysis buffer and nuclear lysis buffer? Anyone can help? Thank you in advance!
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