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Old 04-16-2013, 10:16 PM   #1
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Location: HK

Join Date: Feb 2013
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Default hy PCR is included in library preparation for NGS?

One important step for analyzing whole exome sequencing is to mark duplication so as to decrease miscalling of SNPs.

It may be a silly question, but I am just wondering why PCR is included in library preparation? If this step is skipped, then there's no need to remove duplicates?
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