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Old 07-25-2014, 05:34 AM   #1
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Location: Hannover

Join Date: Sep 2012
Posts: 3
Default PE1 sequence absent in fastq ?

We have observed that in the miseq result fastq files (untrimmed, non-demultiplexed) that a specific adaptor (PE1) is not available in the output data. However, PE2 is always present.

Are there any situations where this can occur ? We are worried if there is a technical problem in our hands, or this could be due to a procedural mishap done in the sequencing facility.

Short: Under which circumstances could the PE1 sequence be absent from the fastq output files ?
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