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Old 10-25-2010, 07:15 AM   #1
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Location: USA

Join Date: Jun 2010
Posts: 8
Default Sequencing for PCR products of different sizes

Hi! Friends

We have constructed directional libraries for RNAseq and we are interested in sequencing a range of PCR products (150-1000 bp) for sequencing. Can anyone tell me how much sample should be submitted for illumina sequencing without affecting cluster density? If concentration is in molar terms, how should I calculate this?

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