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Old 10-16-2018, 06:42 PM   #1
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Default in-situ (in-cell) RT

Hi all, has anyone tried SPLiT-seq as published in "Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding"?

I have specific questions:
(1) How much cells do one usually start with? I can only obtain about 10% of the cells after fixation.
(2) How does one quality check the library? Will the amplicon size distribution be similar to conventional cDNA libraries?

Many thanks in advance! Would be great to know if others are also trying out the same protocol
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