Quick summary,
some library prepped using truseq RNA v2 kit slightly modified protocol (mainly volumes)
Samples 1-8 are library I prepped last week (my first time), some samples at different RNA input conc. (RNA from human blood) PAX gene, and globin cleared (some samples Double globin cleared)
samples 9-11 library prepped few weeks ago by experienced tech using same protocol.
BA was done with a DNA high sensitivity chip
can anyone tell me what they think is going on?
Why are my peaks so big?
Why do a few have a double main peak?
What is the broad trailing peak on some of the samples?
In samples 9-11 I assume early peak is dimers, that is not good right? Dimers will use up some of my reads right?
some library prepped using truseq RNA v2 kit slightly modified protocol (mainly volumes)
Samples 1-8 are library I prepped last week (my first time), some samples at different RNA input conc. (RNA from human blood) PAX gene, and globin cleared (some samples Double globin cleared)
samples 9-11 library prepped few weeks ago by experienced tech using same protocol.
BA was done with a DNA high sensitivity chip
can anyone tell me what they think is going on?
Why are my peaks so big?
Why do a few have a double main peak?
What is the broad trailing peak on some of the samples?
In samples 9-11 I assume early peak is dimers, that is not good right? Dimers will use up some of my reads right?
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