Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • dUTP/UDG or RNA ligation sequencing method?

    Hi community,

    I would like to know the strand specificity type of RNA libraries prepared with Ion Total RNA-Seq Kit v2 - Life Technologies protocol.

    Thank you.

    Regards, Bernardo

  • #2
    See if this has what you need: http://tools.invitrogen.com/content/...s/4476286B.pdf

    Comment


    • #3
      Originally posted by GenoMax View Post
      Thank you GenoMax,

      Which is the difference between dUTP/UDG and RNA ligation sequencing method?

      Bernardo

      Comment


      • #4
        One converts to cDNA and digests away a strand to maintain directional infomration.

        The Ion kit ligates dsAdapters directly to RNA, which typically has better results.

        Comment


        • #5
          Originally posted by snetmcom View Post
          One converts to cDNA and digests away a strand to maintain directional infomration.

          The Ion kit ligates dsAdapters directly to RNA, which typically has better results.

          Thank you snetmcom,

          Now it's clear.

          I found in this paper the dUTP/UDG method explained, althoght the Ion Torrent RNA-seq library preparation is based on ligation method.

          http://www.ncbi.nlm.nih.gov/pubmed/21943893

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM
          • seqadmin
            Techniques and Challenges in Conservation Genomics
            by seqadmin



            The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

            Avian Conservation
            Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
            03-08-2024, 10:41 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 03-27-2024, 06:37 PM
          0 responses
          13 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-27-2024, 06:07 PM
          0 responses
          11 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-22-2024, 10:03 AM
          0 responses
          53 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-21-2024, 07:32 AM
          0 responses
          69 views
          0 likes
          Last Post seqadmin  
          Working...
          X