Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
DEXseq errors Anomilie Bioinformatics 3 11-10-2019 05:31 AM
DEXSeq (1.too many empty 2. paried sample comparison?) senpeng RNA Sequencing 9 06-26-2013 04:03 PM
DEXSeq questions and errors Udmurtia RNA Sequencing 0 02-01-2013 07:49 PM
changing bam files with mixed sample data to per sample mathew Bioinformatics 4 10-11-2012 04:31 AM
DEXSeq sig-regulated exons only high in one sample Pedrissimo RNA Sequencing 3 06-05-2012 02:16 AM

Thread Tools
Old 02-09-2020, 05:53 PM   #1
Location: North Carolina

Join Date: Sep 2011
Posts: 38
Default DEXSeq errors with the sample data

I'm going to use DEXSeq to analyses my PE data (honeybee genome, 3 reps of controls or trt, 3 time-points).

I tried the code for the drosophila sample data, and it showed an error in the end:

Error in gzfile(file, "wb") : cannot open the connection
In addition: Warning message:
In gzfile(file, "wb") :
cannot open compressed file '../data/pasillaDEXSeqDataSet.RData', probable reason 'No such file or directory'

Does this mean it cannot make a plot?

My gene expression data is from Tophat/Cuffdiff, and the splicing.diff file didn't show any hits/significance.

I don't know how to run HTseq or python, but I'm willing to learn more if I need.

Do I need to install python on my computer?

Sorry about these many questions... Thank you so much if you can help me!!!!
bbm is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:55 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO