Hi all,
I have a theoretical question regarding functional annotation:
Is it correct to use raw reads (from NGS) to perform a functional annotation analysis?
Someone suggested me to use raw reads, but I think this idea has no sense, as they are incomplete genes. I have many sequences from RNA-seq and I think contigs are better, as they represent "genes". However, sometimes they match more than one gene…
I will appreciate your opinion,
Thanks in advance,
I have a theoretical question regarding functional annotation:
Is it correct to use raw reads (from NGS) to perform a functional annotation analysis?
Someone suggested me to use raw reads, but I think this idea has no sense, as they are incomplete genes. I have many sequences from RNA-seq and I think contigs are better, as they represent "genes". However, sometimes they match more than one gene…
I will appreciate your opinion,
Thanks in advance,
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