Hi,
I have a question library preparation for MiSeq. I want to sequence the mix of libraries, which length ranges from 300 to 700 bp. I know that sequenator preferably works with shorter sequences. So the question is: In what ratio do I have to mix the libraries of different lengths (ex 300, 500 and 700 bp) so that they have a uniform coverage.
I have a question library preparation for MiSeq. I want to sequence the mix of libraries, which length ranges from 300 to 700 bp. I know that sequenator preferably works with shorter sequences. So the question is: In what ratio do I have to mix the libraries of different lengths (ex 300, 500 and 700 bp) so that they have a uniform coverage.
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