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What are considered "good" ranges for intensity and % alignment?
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GERALD summary information
I am referring to the GERALD summary information and what are considered to be "good" ranges. Is there any documentation on this? I am not running the sample, just receiving the fastq files. -
Thanks
Thanks for all your help.
Some friends at WashU answered my questions.Comment
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I am working with at lab that will be doing pharmacogenetic assays using MiSeq. Getting the genotypes will be straightforward but copy number detection for CYP2D6 is a challenge on any platform because of confusion with CYP2D7. Does anyone know if there is a recommended way of estimating copy numbers with MiSeq?
Thanks,
Don Rule
Translational SoftwareComment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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