Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
DNA extraction methods asis2 Sample Prep / Library Generation 9 06-14-2013 01:02 PM
PubMed: Saliva samples are a viable alternative to blood samples as a source of DNA f Newsbot! Literature Watch 0 03-07-2013 12:20 AM
DNA Extraction from Bone kwaraska Illumina/Solexa 1 11-09-2012 04:33 AM
DNA extraction from saliva coleenmac Sample Prep / Library Generation 5 02-23-2012 12:20 PM
templiphi dna extraction seqgirl123 General 0 10-26-2008 06:50 PM

Thread Tools
Old 05-08-2013, 09:50 AM   #1
Anna IT
Junior Member
Location: Italy

Join Date: May 2013
Posts: 1
Smile DNA extraction from Saliva

Hi everybody!!
I have to extract DNA from Saliva, in order to realize some ION Torrent PGM libraries. I'd like to ask if you know which is the best kit for DNA extraction. In the past we used the Oragene extraction protocol, but our samples were full of proteins. So we have thought about using the DNA mini blood kit, that is compatible also with saliva. In fact our aim is to have a good concentration, but also a great quality of DNA samples. One of the limits of this kit is the cost, because it's really expensive. So I tried to find out something similar, but less expensive. I've read something about the NORGEN DNA saliva isolation kit. Have you ever used it? Do you know other kits that I can use?
Thank you in advance for answering!
Anna IT is offline   Reply With Quote
Old 05-08-2013, 06:56 PM   #2
Location: USA

Join Date: Apr 2013
Posts: 42

My lab always uses the Qiagen DNeasy blood & tissue kit for extracting DNA from tissues. We used it for prepping DNA for Illumina sequencing and it worked well.
jwag is offline   Reply With Quote
Old 05-09-2013, 01:59 AM   #3
james hadfield
Cambridge, UK
Community Forum
Location: Cambridge, UK

Join Date: Feb 2008
Posts: 221

We've made NGS libraries from Oragene DNA and 23andMe used it for my genome. Oragene is expensive but you get masses of high-quality DNA.

here's a list of providers I am aware of:
nexttec 1-Step.
PSP SalivaGene

I'd be intersted to hear if anyone has compared saliva extractions kits.
james hadfield is offline   Reply With Quote
Old 05-16-2013, 07:38 AM   #4
Junior Member
Location: Ottawa, Ontario

Join Date: May 2013
Posts: 1

Hi Anna

We've used Oragene for NGS on both the Ion Torrent and Illumina systems (GAII and HiSeq2000), both targeted and whole genome and we didn't have any problems. We always extract using prepIT-L2P (formerly Oragene Purifier), an EtOH precipitation-based method, and we don't have problems with protein contamination.

Are you assessing protein contamination by 260/280 ratio? If so, you should be able to obtain 1.7 or higher in most cases with a prepIT-L2P extraction. Column-based extraction like prepIT-C2D or various Qiagen kits might give you a bit higher ratio (1.75 or 1.8) but you'll sacrifice yield and, in my experience, it's not necessary to use column extractions for NGS.

Mike Tayeb
Manager, Technical Support
DNA Genotek (manufacturers of Oragene)
MikeTayeb is offline   Reply With Quote
Old 05-16-2013, 07:46 AM   #5
--Site Admin--
Location: SF Bay Area, CA, USA

Join Date: Oct 2007
Posts: 1,358

We've substituted saliva into Qiagen whole blood preps with good results, but be aware of how much bacterial DNA will be in saliva preps.
ECO is offline   Reply With Quote
Old 12-12-2013, 09:01 AM   #6
Chris Slowey
Junior Member
Location: USA

Join Date: Dec 2013
Posts: 1
Default Saliva Devices

Hi guys,

Often times the collection can be the key to getting a pure and high quality sample. I work for a company that produces a range of saliva collection devices, I think you might find the DNA·SAL™ : a perfect fit for gathering a good, clean, and simple sample.

We are a small company really focused on customer service, we are really happy to help anyone through any issues or questions they might have. We go to great lengths to customize our products where there is need as well. Hope this helps.
Chris Slowey is offline   Reply With Quote
Old 12-18-2013, 04:47 AM   #7
Location: SE MN

Join Date: Oct 2013
Posts: 44

There's a NEB kit that removes human DNA for microbiome workflows; NEB has a protocol to elute the human DNA.
ctseto is offline   Reply With Quote

dna, extraction, quality, saliva

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:07 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO