fungal genome completion

[yjpark1091]

Hi guys~

In nowadays, I want to study about fungal genome sequencing based on pacbio sequel system.

Actually i was finished to complete bacterial genome using Pacbio RSII system.

At that time, i was used to check completion of genome assembly using Gepard program for confirm circular chromosome.

In eukaryotic genome (linear chromosome), does any program for check genome assembly completion?

If there are any person who can comment to me, please reply...

[rhall]

While it isn't as simple as looking for circular bacterial chromosomes, if you know the number of chromosomes and rough size distribution you should be able to estimate how complete your assembly is. Although for a true check of how complete the assembly is you would probably need some orthogonal datatype optical / genetic map etc.

[gringer]

One check that can be made for genome completeness is BUSCO:

http://busco.ezlab.org/