I am doing a Raindance project right now. I tried to concatenate the PCR products before fragmentation by Covaris. But the best I can get so far is between 1500bp-2000bp. I was told that PCR products should ligate to more than 5kb, so that Covaris can work well to shear them to 200bp.
I am wondering whether Covaris will still work well with DNA size range 1.5-2kb? Since I really don't know how to improve the ligation...
Thank you very much!
I am wondering whether Covaris will still work well with DNA size range 1.5-2kb? Since I really don't know how to improve the ligation...
Thank you very much!
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